The stereochemistry of the nicotinamide adenine dinucleotide-specific isocitric dehydrogenase reaction.
نویسنده
چکیده
The stereochemistry of the a-ketoglutarate produced in the reaction of nicotinamide adenine dinucleotide-specific isocitric dehydrogenase with threO-D,-iSOCitrk acid-P-T has been determined. The product retained the tritium of the substrate in the p position without inversion. This is similar to the tinding with the nicotinamide adenine dinucleotide phosphate-specific enzyme. The products of the two enzymes are therefore indistinguishable. Experiments with cr-ketoglutarate-/I-T2 provided no evidence for the reversibility of the reaction.
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 241 10 شماره
صفحات -
تاریخ انتشار 1966